Composite

Part:BBa_K3905011:Design

Designed by: Peter Heywood, Harry Weiniger   Group: iGEM21_City_of_London_UK   (2021-09-13)


T7 / gen3 210-3p-517-5p AND-Gate Anti-miRNA / Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Had to ensure that the anti-Mirna alone wouldn't trigger the toehold switch.



Source

This is synthetic, designed in silico, using NUPACK.


References